6FTL
Rubisco from Skeletonema marinoi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.087 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 111.022, 111.022, 396.361 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.600 |
| R-factor | 0.175 |
| Rwork | 0.171 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mz2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.556 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.178 | 0.956 |
| Number of reflections | 75003 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 96.9 | 75.8 |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.05 M HEPES pH 7.5, 0.05 M NaCl, 7-13% PEG 4000 |
