6FTH
HMO binding ABC-transporter associated Solute Binding Protein, Blon_2347 From Bifidobacterium longum infantis ATCC 15697
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.999850 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.399, 70.139, 121.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.838 - 1.469 |
| R-factor | 0.160998932361 |
| Rwork | 0.160 |
| R-free | 0.18677 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3omb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.974 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 1.522 |
| High resolution limit [Å] | 1.468 | 1.469 |
| Rmerge | 0.108 | 0.963 |
| Rmeas | 0.113 | 1.013 |
| Number of reflections | 149050 | 5197 |
| <I/σ(I)> | 16.44 | |
| Completeness [%] | 96.1 | 64.74 |
| Redundancy | 12.6 | 9.6 |
| CC(1/2) | 0.999 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 297 | 0.1M Bis-Tris, 0.2M MgCl2, 25% PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
