6FSW
Structure of Archaeoglobus fulgidus SBDS protein at 1.9 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.810, 44.520, 54.710 |
| Unit cell angles | 75.08, 84.52, 69.72 |
Refinement procedure
| Resolution | 36.708 - 1.900 |
| R-factor | 0.1983 |
| Rwork | 0.197 |
| R-free | 0.23290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t95 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.824 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.710 | 1.770 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 28569 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 94.6 | |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.02M 1,6-Hexanediol; 0.02M 1-Butanol; 0.02M 1,2-Propanediol; 0.02M 2- Propanol; 0.02M 1,4-Butanediol; 0.02M 1,3-Propanediol;0.05 M Imidazole; 0.05 M MES; 12.5% v/v MPD; 12.5% PEG 1000; 12.5% w/v PEG 3350 |
