6FSF

Crystal structure of the tandem PX-PH-domains of Bem3 from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98013, 0.97794, 0.97793
Spacegroup name	P 62
Unit cell lengths	85.370, 85.370, 63.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.685 - 2.200
R-factor	0.2122
Rwork	0.211
R-free	0.23440
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.572
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.700	2.300
High resolution limit [Å]	2.200	2.200
Rmeas	0.047	1.170
Number of reflections	13566	1677
<I/σ(I)>	26.7
Completeness [%]	100.0	99.9
Redundancy	10.1	9.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	reservoir 500 ul 0.1 M PCB pH 7.5-7.7, 17-19% PEG1500; 1ul Bem3 (33-48mg/ml in 20 mM Hepes pH 8.0, 100 mM NaCl, 2mM DTE, 5% glycerol) + 1ul reservoir solution