6FSA
Beta-Cardiac myosin post-rigor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2016-03-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.87290 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.118, 94.319, 125.411 |
| Unit cell angles | 104.76, 92.34, 100.06 |
Refinement procedure
| Resolution | 47.970 - 2.330 |
| R-factor | 0.1913 |
| Rwork | 0.190 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p7h |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.970 | 2.413 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmeas | 0.161 | |
| Number of reflections | 100862 | 10032 |
| <I/σ(I)> | 7.76 | |
| Completeness [%] | 98.5 | 97.87 |
| Redundancy | 3.6 | |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277.15 | 12.5% PEG mix MMW (2k, 3350, 4k, 5k MME), 10% glycerol, 7.5% Sodium Tacsimate pH 6.0, 0.5 mM TCEP |
