6FRY
Photo-Driven Hydrogen Evolution by an Artificial Hydrogenase Utilizing the Biotin-Streptavidin Technology
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 57.615, 57.615, 183.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.800 - 1.700 |
| R-factor | 0.14181 |
| Rwork | 0.139 |
| R-free | 0.20150 |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.409 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 17557 | |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0 |
