6FRQ

Structure of tetragonal Hen Egg-White Lysozyme co-crystallized in presence of 100 mM Tb-Xo4 and 100 mM Potassium sodium tartrate tetrahydrate.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9797
Spacegroup name	P 43 21 2
Unit cell lengths	77.700, 77.700, 38.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.850 - 1.690
R-factor	0.203
Rwork	0.201
R-free	0.23900
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.850
High resolution limit [Å]	1.690
Rpim	0.031
Number of reflections	13611
<I/σ(I)>	13.9
Completeness [%]	99.6
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM sodium acetate pH 4.6, 800 mM sodium chloride, 100 mM sodium and potassium tartrate, 100 mM Tb-Xo4