6FRQ
Structure of tetragonal Hen Egg-White Lysozyme co-crystallized in presence of 100 mM Tb-Xo4 and 100 mM Potassium sodium tartrate tetrahydrate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.700, 77.700, 38.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.850 - 1.690 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.23900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.850 |
| High resolution limit [Å] | 1.690 |
| Rpim | 0.031 |
| Number of reflections | 13611 |
| <I/σ(I)> | 13.9 |
| Completeness [%] | 99.6 |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM sodium acetate pH 4.6, 800 mM sodium chloride, 100 mM sodium and potassium tartrate, 100 mM Tb-Xo4 |
