6FR7
HA1.7 Human T-Cell Receptor specific for Influenza virus epitope PKYVKQNTLKLAT presented by Human Leukocyte Antigen HLA-DR0101
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.350, 49.850, 72.750 |
| Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
| Resolution | 72.530 - 2.310 |
| R-factor | 0.2222 |
| Rwork | 0.219 |
| R-free | 0.27707 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eh8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.938 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.530 | 2.370 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.077 | 0.862 |
| Rmeas | 0.108 | 1.077 |
| Number of reflections | 21302 | 1562 |
| <I/σ(I)> | 9.2 | 2.5 |
| Completeness [%] | 97.1 | 97.3 |
| Redundancy | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2M KSCN, 0.1M Bis-Tris, 20% PEG3350 |
