6FR6
HA1.7 Human T-Cell Receptor specific for Influenza virus epitope PKYVKQNTLKLAT presented by Human Leukocyte Antigen HLA-DR0101
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.000, 50.240, 73.170 |
| Unit cell angles | 90.00, 93.31, 90.00 |
Refinement procedure
| Resolution | 36.520 - 2.980 |
| R-factor | 0.20545 |
| Rwork | 0.201 |
| R-free | 0.29906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eh8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.957 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.520 | 3.060 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.096 | 0.540 |
| Rmeas | 0.132 | 0.736 |
| Number of reflections | 10488 | 782 |
| <I/σ(I)> | 11.6 | 2.3 |
| Completeness [%] | 98.8 | 99.5 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 20% PEG 8K, 0.1 M Tris, 0.2M NH4SO4 |
