6FQR

Crystal structure of IMP3 RRM12 in complex with RNA (CCCC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-30
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9999
Spacegroup name	P 1
Unit cell lengths	30.060, 41.190, 72.270
Unit cell angles	92.14, 100.40, 108.65

Refinement procedure

Resolution	38.823 - 2.100
R-factor	0.1701
Rwork	0.167
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e44
RMSD bond length	0.007
RMSD bond angle	0.796
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (CCP4 7.0.050)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.077	0.541
Number of reflections	17886
<I/σ(I)>	8.1	1.8
Completeness [%]	95.3	93.2
Redundancy	2	2
CC(1/2)	0.994	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	100 mM Bis-Tris pH 6.2, 0.2 M MgCl2, 25% PEG3350