6FQG
GluA2(flop) G724C ligand binding core dimer bound to L-Glutamate (Form A) at 2.34 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.465, 87.655, 68.280 |
| Unit cell angles | 90.00, 110.22, 90.00 |
Refinement procedure
| Resolution | 51.726 - 2.341 |
| R-factor | 0.181217507374 |
| Rwork | 0.179 |
| R-free | 0.23095 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kgc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.262 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 92.400 | 92.400 | 2.030 |
| High resolution limit [Å] | 1.980 | 9.070 | 1.980 |
| Rmerge | 0.064 | 0.011 | 1.267 |
| Rmeas | 0.090 | 0.015 | 1.792 |
| Rpim | 0.064 | 0.011 | 1.267 |
| Total number of observations | 88738 | ||
| Number of reflections | 45174 | 494 | 3142 |
| <I/σ(I)> | 12.9 | ||
| Completeness [%] | 100.0 | 100 | 99.8 |
| Redundancy | 2 | 1.8 | 2 |
| CC(1/2) | 0.987 | 0.999 | 0.230 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 287 | 0.2M Ammonium chloride, 20% PEG 3350, 10uM ZK200775 |
