6FQD

Escherichia Coli Signal Recognition Particle Receptor FtsY NGdN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	I 4
Unit cell lengths	110.963, 110.963, 110.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.278 - 2.100
R-factor	0.179084479123
Rwork	0.177
R-free	0.20833
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fts
RMSD bond length	0.005
RMSD bond angle	0.806
Phasing software	PHASER (1.10.1_2155)
Refinement software	PHENIX (1.12rc0_2798)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.280	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.081	0.430
Rmeas	0.093	0.503
Rpim	0.045	0.253
Number of reflections	38419	3856
<I/σ(I)>	10.34	2.64
Completeness [%]	98.3	98.32
Redundancy	3.9	3.7
CC(1/2)	0.995	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1 M Na-citrate pH 5.6, 20% isopropanol, 20% PEG4000