6FQ0

Crystal structure of the CsuC-CsuA/B chaperone-subunit preassembly complex of the archaic chaperone-usher Csu pili of Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9677
Spacegroup name	H 3
Unit cell lengths	111.419, 111.419, 167.745
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.709 - 2.500
R-factor	0.192
Rwork	0.189
R-free	0.23930
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	83.641	55.710	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge		0.021	0.245
Rmeas	0.049	0.025	0.293
Rpim	0.026	0.013	0.158
Total number of observations	79742	2473	11009
Number of reflections	24003	716	3555
<I/σ(I)>	20.2	48.3	5
Completeness [%]	89.3	84.3	90.2
Redundancy	3.3	3.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M sodium malate, 15% PEG 3350