6FPX

Structure of S. pombe Mmi1 in complex with 11-mer RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 32
Unit cell lengths	105.331, 105.331, 66.706
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.610 - 1.970
R-factor	0.2013
Rwork	0.200
R-free	0.23450
RMSD bond length	0.015
RMSD bond angle	1.415
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.200	2.020
High resolution limit [Å]	1.970	1.970
Rmerge	0.076	0.588
Number of reflections	58703	4049
<I/σ(I)>	8.5	1.6
Completeness [%]	99.8	97
Redundancy	5.1	4.7
CC(1/2)	0.994	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	15% (w/v) PEG 8K, 0.1M CHES pH 9.5