6FPR
Co-translational folding intermediate dictates membrane targeting of the signal recognition particle (SRP)- receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.953723 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.573, 35.487, 31.697 |
| Unit cell angles | 90.00, 96.48, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.650 |
| R-factor | 0.24023 |
| Rwork | 0.238 |
| R-free | 0.27699 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qy9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.880 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.092 | 0.545 |
| Rmeas | 0.099 | 0.586 |
| Number of reflections | 5435 | 781 |
| <I/σ(I)> | 15.1 | 3.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22% MPD NaCacodylate pH=6.2 |
