6FPP
Structure of S. pombe Mmi1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.044, 58.303, 53.769 |
| Unit cell angles | 90.00, 114.73, 90.00 |
Refinement procedure
| Resolution | 51.600 - 1.930 |
| R-factor | 0.1922 |
| Rwork | 0.190 |
| R-free | 0.23820 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.545 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.600 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.078 | 0.459 |
| Number of reflections | 25919 | 1906 |
| <I/σ(I)> | 7.7 | 1.7 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 3.2 | 3.1 |
| CC(1/2) | 0.996 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 20% (w/v) PEG 3350K, 0.1 M Bis-Tris pH 5.5, 0.2 M Magnesium chloride |
