6FPF
Structure of the Ustilago maydis chorismate mutase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 82.768, 83.478, 186.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.740 - 2.200 |
| R-factor | 0.15775 |
| Rwork | 0.155 |
| R-free | 0.20290 |
| Structure solution method | SAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.419 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 1.963 |
| High resolution limit [Å] | 1.895 | 1.895 |
| Rmerge | 0.083 | 0.773 |
| Number of reflections | 63072 | 9715 |
| <I/σ(I)> | 11.1 | 1.8 |
| Completeness [%] | 99.5 | 90.8 |
| Redundancy | 6.7 | 6.3 |
| CC(1/2) | 0.990 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M MES pH 5.0, 20 % (v/v) PEG 6000 |
