6FP7

mTFP1/DARPin 1238_E11 complex in space group P6522

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99986
Spacegroup name	P 65 2 2
Unit cell lengths	99.050, 99.050, 214.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.486 - 1.576
R-factor	0.1486
Rwork	0.148
R-free	0.16390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hqk
RMSD bond length	0.005
RMSD bond angle	0.917
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2131: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.486	1.670
High resolution limit [Å]	1.576	1.576
Rmeas	0.144	1.980
Number of reflections	161477	25309
<I/σ(I)>	13.6	1.3
Completeness [%]	99.3	96.4
Redundancy	13.5	12.9
CC(1/2)	0.999	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M imidazole pH 7.0 and 30% MPD