6FOS
Cyanidioschyzon merolae photosystem I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 163.130, 213.520, 349.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.530 - 4.000 |
| R-factor | 0.379 |
| Rwork | 0.378 |
| R-free | 0.43030 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.829 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((dev_3042: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 4.990 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rpim | 0.036 | 1.236 |
| Number of reflections | 41951 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 80.9 | |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 80mM MgCl2 , 50mM Citric acid, 4-7.5% PEG 4000, 0.025% alpha-DM, pH 4.8-5.0 or 80mM MgCl2 , 50mM Cacodylate, 4-7.5% PEG 4000, 0.025% alpha-DM, pH 5-6 |
