6FON
Elongated conformer of the human copper chaperone for SOD1 complexed with human SOD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 172.481, 172.481, 219.244 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.190 - 3.050 |
| R-factor | 0.20134 |
| Rwork | 0.199 |
| R-free | 0.24628 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fol |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.460 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.190 | 3.210 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.130 | 1.429 |
| Rmeas | 0.135 | 1.481 |
| Rpim | 0.036 | 0.384 |
| Number of reflections | 24090 | 3485 |
| <I/σ(I)> | 18.7 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 14.5 |
| CC(1/2) | 0.999 | 0.693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M MES pH 6.0 0.2 M Magnesium chloride 20 % (w/v) PEG 6000 |
