6FO9
Vitamin D nuclear receptor complex 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0721 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 66.010, 66.010, 264.460 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.856 - 2.701 |
R-factor | 0.1985 |
Rwork | 0.197 |
R-free | 0.22480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hc4 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.739 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1796) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 16314 | |
<I/σ(I)> | 22.4 | |
Completeness [%] | 92.7 | 72.6 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | BisTris pH 6.5, 1.6 M lithium sulfate and 50 mM magnesium sulfate |