6FNZ

Crystal Structure of domain-swapped C-terminal domain of human doublecortin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-12
Detector	PSI PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	P 43 21 2
Unit cell lengths	98.450, 98.450, 114.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.028 - 2.230
R-factor	0.2088
Rwork	0.207
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	modified PDB entry 5ip4
RMSD bond length	0.007
RMSD bond angle	0.922
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12rc1_2801)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.028	44.028	2.230
High resolution limit [Å]	2.180	9.730	2.180
Rmerge	0.086	0.029	4.769
Rmeas	0.092	0.031	5.469
Total number of observations	244277
Number of reflections	29591	416	1456
<I/σ(I)>	12.86	39.31	0.27
Completeness [%]	97.5	97.7	66.5
Redundancy	8.26	6.591	3.909
CC(1/2)	0.999	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	20 mg/mL protein and 3x cmc CHAPS in 20mM CAPS/NaOH pH10.5, 100mM NaCl, 5mM TCEP mixed 60-70% with 40-30% reservoir consisting of 0.1M HEPES/NaOH pH7.0, 10% PEG 5000 MME, 5% Tacsimate