6FNP

Crystal structure of ECF-CbrT, a cobalamin transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99999815963
Spacegroup name	P 1
Unit cell lengths	85.470, 92.860, 105.510
Unit cell angles	72.57, 66.27, 62.89

Refinement procedure

Resolution	47.802 - 3.400
R-factor	0.2458
Rwork	0.238
R-free	0.29340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jsz
RMSD bond length	0.010
RMSD bond angle	1.487
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.802	3.400
High resolution limit [Å]	3.400	3.400
Number of reflections	31753
<I/σ(I)>	0.98
Completeness [%]	88.3
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Potassium chloride 0.1 M Sodium citrate 5.5 37% (v/v) Pentaerythritol propoxylate 5/4 PO/OH, 10% ANAPOE C12E10