6FNO
Caldiarchaeum Subterraneum Ubiquitin:Rpn11-homolog complex, zinc soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.164, 94.832, 171.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.800 - 1.950 |
| R-factor | 0.17795 |
| Rwork | 0.177 |
| R-free | 0.20085 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fnn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.313 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.800 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.081 | 1.030 |
| Number of reflections | 50327 | |
| <I/σ(I)> | 14.4 | 1.7 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 6.9 | 6.88 |
| CC(1/2) | 1.000 | 0.759 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100 mM sodium acetate pH 4.6, 1.5 M ammonium sulfate |
