6FND
Crystal structure of Toxoplasma gondii AKMT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2016-04-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.28225 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.935, 89.371, 91.720 |
| Unit cell angles | 108.69, 101.31, 103.41 |
Refinement procedure
| Resolution | 20.071 - 2.101 |
| R-factor | 0.1921 |
| Rwork | 0.191 |
| R-free | 0.22600 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.702 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.138 | 0.967 |
| Number of reflections | 167741 | 14053 |
| <I/σ(I)> | 15.5 | 2.7 |
| Completeness [%] | 82.8 | 43.2 |
| Redundancy | 2.8 | 2.7 |
| CC(1/2) | 0.990 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 9 | 277 | 0.1 M Tris-HCl (pH 9.0), 0.2 M MgCl2, 30% (w/v) PEG 4,000 |
