6FN8

Domain II of the HUMAN COPPER CHAPERONE FOR SUPEROXIDE DISMUTASE at 1.55 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 43 21 2
Unit cell lengths	68.359, 68.359, 136.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.090 - 1.550
R-factor	0.18645
Rwork	0.185
R-free	0.21687
RMSD bond length	0.005
RMSD bond angle	0.939
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.090	1.550
High resolution limit [Å]	1.550	1.550
Number of reflections	47679
<I/σ(I)>	1.5
Completeness [%]	99.9
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2 M Sodium Chloride, 20% (w/v) PEG 6000; 0.1 M Hepes