6FN0
The animal-like Cryptochrome from Chlamydomonas reinhardtii in complex with 6-4 DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 146.240, 146.240, 67.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.200 - 2.900 |
| R-factor | 0.15915 |
| Rwork | 0.157 |
| R-free | 0.21229 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.507 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.400 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.050 | |
| Number of reflections | 16737 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 278 | 0.1 M MES, pH 5.5, 10 % (w/v) PEG 6000 |
