6FMP
Keap1 - peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-11-22 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.409, 76.259, 208.284 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.922 - 2.920 |
| R-factor | 0.1835 |
| Rwork | 0.181 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zgd |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.496 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.020 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Rmerge | 0.625 | |
| Number of reflections | 26886 | 2360 |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 98.9 | 92.3 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 4.0 M ammonium acetate, 0.1 M sodium acetate trihydrate pH 4.6 |
