6FMJ

pVHL:EloB:EloC in complex with (2S,4R)-1-((S)-2-Acetamidopropanethioyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl) benzyl)pyrrolidine-2-carboxamide (ligand 3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 41 2 2
Unit cell lengths	93.518, 93.518, 364.314
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.970 - 2.450
R-factor	0.2155
Rwork	0.213
R-free	0.26541
RMSD bond length	0.005
RMSD bond angle	1.234
Data reduction software	XDS
Data scaling software	Aimless (data scaling version: 0.5.9)
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	2.510
High resolution limit [Å]	2.450	2.450
Number of reflections	60839
<I/σ(I)>	10.6
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 3350, MgOAc, Sodium cacodylate, DTT