6FMG

Structure of the Mannose Transporter IIA Domain from Streptococcus pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	P 63
Unit cell lengths	138.528, 138.528, 69.767
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.179 - 1.800
R-factor	0.1716
Rwork	0.170
R-free	0.19650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pdo
RMSD bond length	0.011
RMSD bond angle	1.088
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.150	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.147	2.052
Number of reflections	1461081
<I/σ(I)>	14.5
Completeness [%]	100.0	100
Redundancy	20.6	10.5
CC(1/2)	0.999	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Magnesium acetate tetrahydrate 0.1 M Sodium cacodylate 6.5 20 % w/v PEG 8000