6FME

Structure of sucrose phosphorylase from Bifidobacterium adolescentis bound to glycosylated resveratrol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	76.010, 101.925, 152.569
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.506 - 1.510
R-factor	0.1667
Rwork	0.166
R-free	0.18840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.506	1.564
High resolution limit [Å]	1.510	1.510
Rmerge	0.081	1.230
Number of reflections	175921
<I/σ(I)>	10.32	0.89
Completeness [%]	95.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	PEG 1000, 150MM NACL, MES pH7