6FM8
RPAP3 c-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 39.631, 39.631, 70.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.540 - 1.780 |
| R-factor | 0.233 |
| Rwork | 0.231 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | helix |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | Arcimboldo |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.400 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.113 | 0.870 |
| Rpim | 0.039 | 0.297 |
| Number of reflections | 5844 | 418 |
| <I/σ(I)> | 8.9 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.7 | 9 |
| CC(1/2) | 0.999 | 0.505 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 287 | 0.5M Potassium thiocyanate, 0.1M Bis-Tris Propane pH 7.0 |
