6FM3
Deoxyguanylosuccinate synthase (DgsS) structure with ADP at 1.9 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-01-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.780, 58.200, 71.960 |
| Unit cell angles | 90.00, 102.44, 90.00 |
Refinement procedure
| Resolution | 48.320 - 1.950 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.25200 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.320 | 2.440 |
| High resolution limit [Å] | 1.900 | 2.300 |
| Number of reflections | 24769 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 93.7 | |
| Redundancy | 5.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 2M NaCl 12%w/v PEG 6K |
