6FM2

CARP domain of mouse cyclase-associated protein 1 (CAP1) bound to ADP-actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 61 2 2
Unit cell lengths	73.833, 73.833, 453.377
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.570 - 2.800
R-factor	0.186
Rwork	0.183
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4z 3tpq
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.340	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.080	1.371
Rmeas	0.087	1.494
Rpim	0.035	0.585
Number of reflections	19109	2749
<I/σ(I)>	11.6	1.3
Completeness [%]	98.2	99.9
Redundancy	6	6.4
CC(1/2)	0.999	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl, 0.2 M LiCl, 20% (w/v) PEG8000