6FLS
Pentapeptide repeat family protein from Clostridium botulinum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.751, 108.475, 112.890 |
| Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
| Resolution | 112.530 - 2.800 |
| R-factor | 0.1716 |
| Rwork | 0.170 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.357 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.530 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 63384 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-Tris pH 5.5, 25% PEG 3350 |
