6FLR
Super-open structure of the AMPAR GluA3 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.196, 105.145, 134.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.770 - 2.510 |
| R-factor | 0.22019 |
| Rwork | 0.218 |
| R-free | 0.25714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o21 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.378 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.770 | 2.570 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Number of reflections | 26508 | |
| <I/σ(I)> | 11.7 | 1.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.1 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 18% PEG3350 and 0.2 M ammonium citrate |
