6FLI
The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - Co-crystallization with 4-bromo cinnamic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2016-08-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 222.690, 112.660, 144.830 |
| Unit cell angles | 90.00, 111.21, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.22500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.320 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 66506 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 150mM MgCl2 100mM Tris 8.0 35% PEG 200 |
