6FLB

3H5 Fab bound to EDIII of DenV 2 Xtal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	38.492, 131.573, 139.442
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.290 - 2.200
R-factor	0.1776
Rwork	0.175
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.11.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.300	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	37183
<I/σ(I)>	14.8
Completeness [%]	100.0
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% w/v PEG 3350, 0.2 M Ammonium di Hydrogen Phosphate using a protein concentration of 6 mg/ml.