6FLA
3H5 Fab bound to EDIII of DenV 2 Xtal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.951 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.840, 263.870, 272.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.590 - 2.900 |
| R-factor | 0.2042 |
| Rwork | 0.203 |
| R-free | 0.22110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 33359 | |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% w/v PEG 3350, 0.2 M di-Sodium Tartrate at a protein concentration of 4.16 mg/ml |
