6FL9
The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - Wild type 2.3 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2015-12-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 219.600, 112.960, 144.430 |
| Unit cell angles | 90.00, 112.05, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.23600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.340 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 144663 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 290 | 100 mM Tris pH 8.0; 150 mM MgCl2; 3% DMSO; 32% PEG 200 |
