6FKR

Crystal structure of the dolphin proline-rich antimicrobial peptide Tur1A bound to the Thermus thermophilus 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972422
Spacegroup name	P 21 21 21
Unit cell lengths	209.680, 449.250, 621.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.757 - 3.200
R-factor	0.1929
Rwork	0.190
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f8k
RMSD bond length	0.030
RMSD bond angle	1.978
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.757	49.757	3.280
High resolution limit [Å]	3.200	14.310	3.200
Rmerge	0.393	0.117	1.902
Rmeas	0.423	0.126	2.045
Total number of observations	6646089
Number of reflections	941634	10701	69402
<I/σ(I)>	4.08	13.04	1
Completeness [%]	98.6	93.7	98.6
Redundancy	7.058	6.981	7.246
CC(1/2)	0.980	0.988	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	Tris-HCl pH 7.6, PEG 20,000, 2-methyl-2,4-pentanediol, L-arginine