6FK7

Crystal structure of N2C/D282C stabilized opsin bound to RS06

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	243.391, 243.391, 111.547
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.647 - 2.620
R-factor	0.2369
Rwork	0.236
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j4q
RMSD bond length	0.004
RMSD bond angle	0.657
Data reduction software	XDS ((VERSION Oct 15)
Data scaling software	Aimless
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	2.720
High resolution limit [Å]	2.620	2.620
Rmerge	0.199	1.020
Number of reflections	37925
<I/σ(I)>	9.62	0.66
Completeness [%]	100.0	100
Redundancy	11.53	11.92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	AMMONIUM SULPHATE, SODIUM ACETATE, D(+)-TREHALOSE, PH 6.0