6FJY

Crystal structure of CsuC-CsuE chaperone-tip adhesion subunit pre-assembly complex from archaic chaperone-usher Csu pili of Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	53.836, 63.854, 89.245
Unit cell angles	74.65, 79.65, 69.07

Refinement procedure

Resolution	53.925 - 2.310
R-factor	0.2185
Rwork	0.216
R-free	0.27170
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.925	53.925	2.430
High resolution limit [Å]	2.310	7.300	2.310
Rmerge		0.029	0.792
Rmeas	0.064	0.034	0.935
Rpim	0.033	0.017	0.489
Total number of observations	166711	5070	22911
Number of reflections	44881	1400	6462
<I/σ(I)>	13	39.5	1.5
Completeness [%]	96.0	94.7	94.3
Redundancy	3.7	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.1 M bis-Tris, 17% PEG 3350
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.1 M bis-Tris, 17% PEG 3350