6FJV

Rpn11 homolog from Caldiarchaeum Subterraneum, truncated

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	38.954, 38.954, 194.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.200 - 1.350
R-factor	0.17264
Rwork	0.171
R-free	0.21047
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fju
RMSD bond length	0.015
RMSD bond angle	1.732
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.200	1.430
High resolution limit [Å]	1.350	1.350
Rmerge	0.066	1.110
Number of reflections	34313
<I/σ(I)>	21.1	2.25
Completeness [%]	100.0	99.9
Redundancy	12.4	11.5
CC(1/2)	0.999	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	100 mM sodium acetate pH 4.6, 1.5 M ammonium sulfate, 25% PEG 4000