6FJP

Adenovirus species 26 knob protein, high resolution, High pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 3
Unit cell lengths	85.717, 85.717, 85.717
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.610 - 1.090
R-factor	0.16787
Rwork	0.167
R-free	0.17664
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knb
RMSD bond length	0.017
RMSD bond angle	1.891
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.610	1.140
High resolution limit [Å]	1.090	1.090
Rmerge	0.068	0.921
Rmeas	0.069	0.951
Number of reflections	88303	12829
<I/σ(I)>	21.7	2.6
Completeness [%]	100.0	100
Redundancy	21	16.5
CC(1/2)	1.000	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	PACT Premier A04: 0.1 M SPG, 25% PEG 1500