6FJO

Adenovirus species 26 knob protein, very high resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 3
Unit cell lengths	85.776, 85.776, 85.776
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.650 - 1.170
R-factor	0.16992
Rwork	0.169
R-free	0.19018
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knb
RMSD bond length	0.021
RMSD bond angle	2.080
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.780	1.230
High resolution limit [Å]	1.170	1.170
Rmerge	0.065	1.370
Rmeas	0.066	1.404
Number of reflections	71878	10438
<I/σ(I)>	24.8	2.3
Completeness [%]	100.0	100
Redundancy	21.8	21.2
CC(1/2)	1.000	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	PACT premier A01: 0.1 M SPG buffer, 25% PEG 1500