6FJO
Adenovirus species 26 knob protein, very high resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 85.776, 85.776, 85.776 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.650 - 1.170 |
| R-factor | 0.16992 |
| Rwork | 0.169 |
| R-free | 0.19018 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1knb |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.080 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.780 | 1.230 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.065 | 1.370 |
| Rmeas | 0.066 | 1.404 |
| Number of reflections | 71878 | 10438 |
| <I/σ(I)> | 24.8 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.8 | 21.2 |
| CC(1/2) | 1.000 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | PACT premier A01: 0.1 M SPG buffer, 25% PEG 1500 |
