6FJN

Adenovirus species 26 knob protein, 0.97A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 3
Unit cell lengths	86.012, 86.012, 86.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.820 - 0.970
R-factor	0.18279
Rwork	0.182
R-free	0.19478
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knb
RMSD bond length	0.017
RMSD bond angle	1.936
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.820	1.000
High resolution limit [Å]	0.970	0.970
Rmerge	0.043	0.745
Rmeas	0.045	0.975
Number of reflections	118603	4055
<I/σ(I)>	27.3	0.7
Completeness [%]	94.9	43.9
Redundancy	16.7	1.6
CC(1/2)	1.000	0.427

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MMT 25% PEG 1500