6FIX
antitoxin GraA in complex with its operator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2015-04-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 |
| Unit cell lengths | 105.550, 105.550, 149.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.853 - 3.800 |
| R-factor | 0.248 |
| Rwork | 0.246 |
| R-free | 0.28570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.076 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.853 | 3.929 |
| High resolution limit [Å] | 3.793 | 3.793 |
| Rmerge | 0.127 | 1.692 |
| Number of reflections | 18427 | 1797 |
| <I/σ(I)> | 8.84 | 0.91 |
| Completeness [%] | 99.5 | 95.69 |
| Redundancy | 5.2 | 5.1 |
| CC(1/2) | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M lithium sulfate, 0.1 M sodium acetate pH 4.5, 50% PEG 400 |
