6FIV
STRUCTURAL STUDIES OF HIV AND FIV PROTEASES COMPLEXED WITH AN EFFICIENT INHIBITOR OF FIV PR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-11 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.760, 50.760, 73.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.155 * |
| R-free | 0.26600 * |
| Structure solution method | OTHER |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.024 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | X-PLOR |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.372 |
| Number of reflections | 8437 | |
| <I/σ(I)> | 1.98 | |
| Completeness [%] | 93.1 * | 75 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * | SAMPLE: 3.4MG/ML Q99V IN 25MM IMIDAZOL BUFFER AT PH 7.0 WITH 1MM EDTA AND 10MM DTT. WELL SOLUTION: 1.6M AMMONIUM SULFATE IN SODIUM ACETATE BUFFER AT PH 4.5 MIXING SAMPLE AND WELL SOLLUTION 1:1, pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | TL-3 | 7 (mg/ml) | |
| 2 | 1 | drop | imidazole-HCl | 50 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 2 (M) | |
| 6 | 1 | drop | sodium acetate | 0.1 (M) | |
| 7 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 8 | 1 | reservoir | sodium acetate | 0.1 (M) |
