6FI2
VexL: A periplasmic depolymerase provides new insight into ABC transporter-dependent secretion of bacterial capsular polysaccharides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972422 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 94.680, 94.680, 78.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.600 - 1.220 |
| R-factor | 0.14038 |
| Rwork | 0.139 |
| R-free | 0.16621 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.561 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.600 | 1.240 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Number of reflections | 106029 | 5241 |
| <I/σ(I)> | 17 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 12.7 |
| CC(1/2) | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.58 M sodium malonate, 0.1 M BisTris HCl pH 6.5, 0.13 M ammonium citrate, and 2.5 % (v/v) 2-methyl-2,4-pentanediol |
