6FI2

VexL: A periplasmic depolymerase provides new insight into ABC transporter-dependent secretion of bacterial capsular polysaccharides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.972422
Spacegroup name	P 42 21 2
Unit cell lengths	94.680, 94.680, 78.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.600 - 1.220
R-factor	0.14038
Rwork	0.139
R-free	0.16621
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.561
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	BALBES
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.600	1.240
High resolution limit [Å]	1.220	1.220
Number of reflections	106029	5241
<I/σ(I)>	17	2.1
Completeness [%]	100.0	100
Redundancy	12.8	12.7
CC(1/2)		0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.58 M sodium malonate, 0.1 M BisTris HCl pH 6.5, 0.13 M ammonium citrate, and 2.5 % (v/v) 2-methyl-2,4-pentanediol